Tottenham will play all their home games at Wembley Stadium next season, the club have announced.
Spurs will move into the national stadium for the 2017/18 campaign while work on their new 61,000-seater ground is completed.
It means Sunday's Premier League match at home to Arsenal will be the last north London derby at White Hart Lane, while Manchester United will be their final opponents there on May 14.
Tottenham, who have played at White Hart Lane since 1899, last month extended the deadline for a final decision on Wembley but triggered the option already agreed with the Football Association on Friday.
Chairman Daniel Levy said: "The Lane means a huge amount to each and every one of us and we needed to gain greater certainty on the delivery of the new stadium before we made the final decision to commence with the decommissioning of our iconic, historic home for some 118 years.
"We shall ensure that we give the Lane a fitting farewell when we play our last match here on May 14.
"Wembley will be our home for a season and then we shall return to what will be one of the best stadiums of its kind and the most unique in the world playing host to NFL games too - a stadium that will be key to our future growth and success.
"Both on and off the pitch this is an incredibly exciting time in the history of our club."